Evolutionary genomics of the cold-adapted diatom Fragilariopsis cylindrus

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Building a locally diploid genome and transcriptome of the diatom Fragilariopsis cylindrus

The genome of the cold-adapted diatom Fragilariopsis cylindrus is characterized by highly diverged haplotypes that intersperse its homozygous genome. Here, we describe how a combination of PacBio DNA and Illumina RNA sequencing can be used to resolve this complex genomic landscape locally into the highly diverged haplotypes, and how to map various environmentally controlled transcripts onto ind...

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ژورنال

عنوان ژورنال: Nature

سال: 2017

ISSN: 0028-0836,1476-4687

DOI: 10.1038/nature20803